Ribozyme相关论文
选择小鼠腺苷脱氨酶mRNA做为靶RNA系统，采用计算机辅助设计，人工设计了能特异性定点切割小鼠腺苷脱氨酶mRNA的Ribozyme，命名为ADARb26......
Effects of ribozyme targeting platelet-derived growth factor receptor β subunit gene on the prolifer
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Activity identification of anti-caspase-3 mRNA hammerhead ribozyme in both cell-free condition and B
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According to the information on the hammerhead structure of ribozyme known so far,thecleavage occurs at the 3′-side of ......
构建了由RSV-LTR启动子带动并能在细胞内稳定复制的Ribozyme的自身修剪表达质粒pRSV-Rz523、Ribozyme反义对照质粒pRSV-AE7及人增......
Effects of NHE-1 ribozyme gene transfection on apoptosis of rat pulmonary artery smooth muscle cells
Objective:To investigate the effects of the transfection of NHE-1 ribozyme gene on the apoptosis of pulmonary artery smo......
Comparison of cleavage activity in vitro between two ribozymes targeted against different sites of P
Objective: To compare the cleavage activity of ribozymes directed against 2 sites of PDGF receptorβ subunit cDNA gene i......
Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing ce
Objective: To acquire a ribozyme against the E6 gene of human papillomaviruses type 16 (HPV16E6) and investigate its eff......
AIM: To prepare and identify specific anti-mouse caspase12 hammerhead ribozymes in vitro, in order to select a more effe......
Construction of retroviral vector of p125FAK specific ribozyme genes and its effects on BGC-823 cell
AIM: To construct the retroviral vector of p125FAK specific ribozyme genes and to explore the feasibility of ribozyme in......
c-erbB-2原癌基因的产物是跨膜生长因子受体的一种,具有内在酪氨激酶活性,在肿瘤细胞生长信号传递中起着重要的作用.已发现该基因......
多药耐药MDRl基因的过度表达是导致肿瘤细胞产生MDR的重要机制.c-fos基因表达的变化很可能对MDRl基因的表达起着调控作用.为逆转肝......
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目前,就Ribozyme的译名议论颇多。本人以为这仅是我国的文字、语言及文化如何与西方文字,语言及文化进行交流甚至交融的大问题在......
利用ribozyme专一水解RNA的特性,设计ribozyme定点切割基因表达的中间物mRNA以及病毒RNA,可以阻断基因表达和抑制病毒复制,从而达......
在当今基因干预和基因治疗领域里,有两项极为引人注目的技术,那就是反义核酸技术和ribozyme技术。而二者的完美结合,则成为基因治......
Ribozyme具有催化切割其他RNA分子的能力,不少研究者针对这一特点,试图通过人工设计的Ribozyme破坏致病的RNA分子,在爱滋病、慢性......
Ｒｉｂｏｚｙｍｅ是具有广泛催化活性的ＲＮＡ分子，对其催化裂解活性的深入研究及二级结构模式的提出推动了人工设计核酶及其应用研究进程。本文还结合最近......
通过体外转录结合乙醇沉淀，建立了体外大量制备抗ＨＰＶ１６Ｅ７－Ｒｚ的方法。切割实验表明，ｒｉｂｏｚｙｍｅ可在体外准确和有效地切割Ｅ７靶ＲＮＡ，形成８５ｎｔ和８６ｎｔ大小的切割产物。体外制备......
应用计算机分析了抗ＨＰＶ１６Ｅ７－ｒｉｂｏｚｙｍｅ的体外切割反应结果，发现ｒｉｂｏｚｙｍｅ和靶ＲＮＡ的二级结构及其能量变化能影响ｒｉｂｏｚｙｍｅ的切割活性。根据锤头结构ｒｉｂｏｚｙｍｅ的能量变化模型，对抗ＨＰＶ１６Ｅ７－ｒｉｂｏｚｙｍｅ和靶ＲＮＡ在......
锤头结构Ribozyme切割底物时的催化结构包括3个螺旋区和中间10核苷酸的单链区(编号为3～9,12～14,在我们过去文章中称3个双链区为茎区,......
目的构建以济南本土分离株为模板的EV71感染性克隆,转染细胞以获得拯救病毒并观察其生物学特性,为研究EV71病毒基因结构和功能奠定......
目的:观察针对HBV C 区双位点核酶对2 .2 .15 细胞中HBEAG 表达的抑制作用.方法:利用亚克隆技术,从质粒P GEM － RZ123 切下ECOR I－ B......
八十年代初 ,由美国科学家 T Cech和 S Alt-man发现了 Ribozyme(核酶 ) ,随着人类基因工程研究的深入 ,部分临床实验工作者和基础......
人们一直认为酶是唯一的生物催化剂,酶和生物催化剂概念具有等价性.Ribozyme的发现打破了这种等价性,生物催化剂上升为属概念,酶和......
人类乳头状瘤病毒(Human Papillomavirus,HPV)通过感染宫颈上皮细胞,其DNA片段整合到宿主细胞基因组中,表达产物结合和降解p53、Rb......
To detect a new and more effective way against apoptosis mouse lymphomatic cell lineYac-1 in which fas gene was expresse......
GENE THERAPY USING RETROVIRAL VECTOR OF bcr-abl SPECIFIC MULTI-UNIT RIBOZYMES COULD INHIBIT CML CELL
Objective: To investigate the in vitro cleavage ability and effects on apoptosis and cell growth of the bcr-abl fusion g......
To reverse multidrug resistance(MDR) of HepG2 by anti-MDR1 hammerhead ribozyme,an anti-MDR1 hammerhead ribozyme was deve......
Effects of blocking androgen receptor expression with specific hammerhead ribozyme on in vitro growt
Objective To study the possibility of gene therapy for prostate cancer by blocking androgen receptor (AR) gene expressio......
Effects of ribozyme targeting platelet-derived growth factor receptor β subunit gene on the prolifer
Background Activation and proliferation of hepatic stellate cells (HSC) is essentially involved in the development and p......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
背景与目的:肿瘤细胞可表达FasL,从而诱导表达Fas的T细胞凋亡,即肿瘤细胞的“Fas反击作用”.本研究通过检测抗Fas核酶对小鼠T细胞......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Telomerase plays an important role in cell proliferation and carcinogenesis and is believed to be a good target for anti......
背景与目的:肿瘤细胞可表达FasL,从而诱导表达Fas的T细胞凋亡,即肿瘤细胞的"Fas反击作用".本研究通过检测抗Fas核酶对小鼠T细胞凋......
Effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on proliferation of vascular smoo
Ojective: To investigate the effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on the proliferation of ......
Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing ce
Objective: To acquire a ribozyme against the E6 gene of human papillomaviruses type 16 (HPV16E6) and investigate its eff......
EFFECTS OF IN VIVO GENE TRANSDUCTION OF ANTI-MDR1 RIBOZYME IN COMBINATION WITH CHEMOTHERAPY ON MULTI
Objective: To study the effect of adenovirus- mediated transfer of anti-MDR1 ribozyme on the reversal of multidrug resis......
Cisplatin sensitivity and mechanisms of anti-HPV16 E6-ribozyme on cervical carcinoma CaSKi cell line
Objective: The aim of this study was to study the cisplatin sensitizing effect and mechanism of anti-HPV16 E6- ribozyme ......
AIM: To prepare and identify specific anti-mouse caspase12 hammerhead ribozymes in vitro, in order to select a more effe......
Effects of ribozyme targeting platelet-derived growth factor receptor β subunit gene on the prolifer
Background Activation and proliferation of hepatic stellate cells (HSC) is essentially involved in the development and p......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
